Scooby-Doo and his friends once again face the mystery and disturbing events at the Natural History Museum. Exhibits and artifacts have been mixed up and damaged by the mysterious Glowing Bug Man.

The game's story begins in the main hall of the museum, where Scooby, Shaggy, Daphne, Velma and Fred will have to solve subsequent puzzles. The control is carried out using the mouse; when the cursor takes the form of a pointing finger, you can move in a given direction; when it turns into a paw, you can pick up or move items or click on an item to see animations.

Your task is to collect clues by listening to tapes (found in different places) and solve puzzles. For each solved puzzle, you will receive an item, which you place in the files under the paintings of five museum employees. Thanks to this method, you can eliminate suspects until you find the perpetrator of all the chaos. Puzzle-solving involves moving and matching patterned tiles, completing rocks, remembering which buttons on the ice cream machine correspond to which flavors, and much more. Throughout the game, the emphasis is on pattern recognition and logical thinking.